recombinant wt ape1 Search Results


recombinant wt ape1  (OriGene)
90
OriGene recombinant wt ape1
Recombinant Wt Ape1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+wt+ape1/APE1+(APEX1)+(NM_001641)+Human+Recombinant+Protein/pmc05093586__srep36125___s1-6-19-23
Average 90 stars, based on 1 article reviews
recombinant wt ape1 - by Bioz Stars, 2026-10
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e coli udg  (New England Biolabs)
97
New England Biolabs e coli udg
Effect of HBR deletion on uracil removal by <t>UDG-APE1.</t> 601 DNA, containing a single uracil at the specified locations, was 5′-end–labeled with either [γ-32P]ATP or Cy3 and annealed with the corresponding undamaged complementary strand. Substrates were reconstituted with WT <t>or</t> <t>ΔHBR</t> histone octamers, and DNA cleavage reactions were performed at 37 °C for the specified time with UDG (30 nm) and APE1 (10 nm). Cleavage activity was measured on denaturing gels (see insets), as described under “Experimental procedures.” The data points represent the means ± S.D. of at least three independent experiments.
E Coli Udg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+wt+ape1/APE+1/pmc05949990-328-21-27
Average 97 stars, based on 1 article reviews
e coli udg - by Bioz Stars, 2026-10
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q5 site-directed mutagenesis kit  (New England Biolabs)
99
New England Biolabs q5 site-directed mutagenesis kit
Effect of HBR deletion on uracil removal by <t>UDG-APE1.</t> 601 DNA, containing a single uracil at the specified locations, was 5′-end–labeled with either [γ-32P]ATP or Cy3 and annealed with the corresponding undamaged complementary strand. Substrates were reconstituted with WT <t>or</t> <t>ΔHBR</t> histone octamers, and DNA cleavage reactions were performed at 37 °C for the specified time with UDG (30 nm) and APE1 (10 nm). Cleavage activity was measured on denaturing gels (see insets), as described under “Experimental procedures.” The data points represent the means ± S.D. of at least three independent experiments.
Q5 Site Directed Mutagenesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+wt+ape1/Q5+Site-Directed+Mutagenesis+Kit/custom%40e0552%4036037088
Average 99 stars, based on 1 article reviews
q5 site-directed mutagenesis kit - by Bioz Stars, 2026-10
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odyssey  (LI-COR)
99
LI-COR odyssey
Effect of HBR deletion on uracil removal by <t>UDG-APE1.</t> 601 DNA, containing a single uracil at the specified locations, was 5′-end–labeled with either [γ-32P]ATP or Cy3 and annealed with the corresponding undamaged complementary strand. Substrates were reconstituted with WT <t>or</t> <t>ΔHBR</t> histone octamers, and DNA cleavage reactions were performed at 37 °C for the specified time with UDG (30 nm) and APE1 (10 nm). Cleavage activity was measured on denaturing gels (see insets), as described under “Experimental procedures.” The data points represent the means ± S.D. of at least three independent experiments.
Odyssey, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+wt+ape1/Odyssey/custom%40Odyssey%4030509560
Average 99 stars, based on 1 article reviews
odyssey - by Bioz Stars, 2026-10
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odyssey imaging system  (LI-COR)
99
LI-COR odyssey imaging system
Effect of HBR deletion on uracil removal by <t>UDG-APE1.</t> 601 DNA, containing a single uracil at the specified locations, was 5′-end–labeled with either [γ-32P]ATP or Cy3 and annealed with the corresponding undamaged complementary strand. Substrates were reconstituted with WT <t>or</t> <t>ΔHBR</t> histone octamers, and DNA cleavage reactions were performed at 37 °C for the specified time with UDG (30 nm) and APE1 (10 nm). Cleavage activity was measured on denaturing gels (see insets), as described under “Experimental procedures.” The data points represent the means ± S.D. of at least three independent experiments.
Odyssey Imaging System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+wt+ape1/Odyssey+Imaging+System/custom%40odyssey-imaging-system%4030509560
Average 99 stars, based on 1 article reviews
odyssey imaging system - by Bioz Stars, 2026-10
99/100 stars
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wt c76a c-terminal his-tag exog  (GenScript corporation)
90
GenScript corporation wt c76a c-terminal his-tag exog
Effect of HBR deletion on uracil removal by <t>UDG-APE1.</t> 601 DNA, containing a single uracil at the specified locations, was 5′-end–labeled with either [γ-32P]ATP or Cy3 and annealed with the corresponding undamaged complementary strand. Substrates were reconstituted with WT <t>or</t> <t>ΔHBR</t> histone octamers, and DNA cleavage reactions were performed at 37 °C for the specified time with UDG (30 nm) and APE1 (10 nm). Cleavage activity was measured on denaturing gels (see insets), as described under “Experimental procedures.” The data points represent the means ± S.D. of at least three independent experiments.
Wt C76a C Terminal His Tag Exog, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+wt+ape1/wt+c76a+c+terminal+his+tag+exog/pmc05093586__srep36125___s1-6-27-34
Average 90 stars, based on 1 article reviews
wt c76a c-terminal his-tag exog - by Bioz Stars, 2026-10
90/100 stars
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n a plko 1 trc cloning vector moffat  (Addgene inc)
96
Addgene inc n a plko 1 trc cloning vector moffat
Effect of HBR deletion on uracil removal by <t>UDG-APE1.</t> 601 DNA, containing a single uracil at the specified locations, was 5′-end–labeled with either [γ-32P]ATP or Cy3 and annealed with the corresponding undamaged complementary strand. Substrates were reconstituted with WT <t>or</t> <t>ΔHBR</t> histone octamers, and DNA cleavage reactions were performed at 37 °C for the specified time with UDG (30 nm) and APE1 (10 nm). Cleavage activity was measured on denaturing gels (see insets), as described under “Experimental procedures.” The data points represent the means ± S.D. of at least three independent experiments.
N A Plko 1 Trc Cloning Vector Moffat, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+wt+ape1/pLKO%2E1+-+TRC+cloning+vector+(Plasmid+%2310878)/pm37703179-427-232-239
Average 96 stars, based on 1 article reviews
n a plko 1 trc cloning vector moffat - by Bioz Stars, 2026-10
96/100 stars
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pet15b merck millipore  (Addgene inc)
93
Addgene inc pet15b merck millipore
Effect of HBR deletion on uracil removal by <t>UDG-APE1.</t> 601 DNA, containing a single uracil at the specified locations, was 5′-end–labeled with either [γ-32P]ATP or Cy3 and annealed with the corresponding undamaged complementary strand. Substrates were reconstituted with WT <t>or</t> <t>ΔHBR</t> histone octamers, and DNA cleavage reactions were performed at 37 °C for the specified time with UDG (30 nm) and APE1 (10 nm). Cleavage activity was measured on denaturing gels (see insets), as described under “Experimental procedures.” The data points represent the means ± S.D. of at least three independent experiments.
Pet15b Merck Millipore, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+wt+ape1/MP-P+(Plasmid+%2369661)/pm31995729-261-268-265
Average 93 stars, based on 1 article reviews
pet15b merck millipore - by Bioz Stars, 2026-10
93/100 stars
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2016 addgene plasmid  (Addgene inc)
93
Addgene inc 2016 addgene plasmid
Effect of HBR deletion on uracil removal by <t>UDG-APE1.</t> 601 DNA, containing a single uracil at the specified locations, was 5′-end–labeled with either [γ-32P]ATP or Cy3 and annealed with the corresponding undamaged complementary strand. Substrates were reconstituted with WT <t>or</t> <t>ΔHBR</t> histone octamers, and DNA cleavage reactions were performed at 37 °C for the specified time with UDG (30 nm) and APE1 (10 nm). Cleavage activity was measured on denaturing gels (see insets), as described under “Experimental procedures.” The data points represent the means ± S.D. of at least three independent experiments.
2016 Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+wt+ape1/pNCS-mClover3+(Plasmid+%2374236)/pm31995729-261-256-257
Average 93 stars, based on 1 article reviews
2016 addgene plasmid - by Bioz Stars, 2026-10
93/100 stars
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xmni  (New England Biolabs)
95
New England Biolabs xmni
Effect of HBR deletion on uracil removal by <t>UDG-APE1.</t> 601 DNA, containing a single uracil at the specified locations, was 5′-end–labeled with either [γ-32P]ATP or Cy3 and annealed with the corresponding undamaged complementary strand. Substrates were reconstituted with WT <t>or</t> <t>ΔHBR</t> histone octamers, and DNA cleavage reactions were performed at 37 °C for the specified time with UDG (30 nm) and APE1 (10 nm). Cleavage activity was measured on denaturing gels (see insets), as described under “Experimental procedures.” The data points represent the means ± S.D. of at least three independent experiments.
Xmni, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+wt+ape1/XmnI/custom%40r0194%4026743004
Average 95 stars, based on 1 article reviews
xmni - by Bioz Stars, 2026-10
95/100 stars
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nt.bstnbi  (New England Biolabs)
96
New England Biolabs nt.bstnbi
Effect of HBR deletion on uracil removal by <t>UDG-APE1.</t> 601 DNA, containing a single uracil at the specified locations, was 5′-end–labeled with either [γ-32P]ATP or Cy3 and annealed with the corresponding undamaged complementary strand. Substrates were reconstituted with WT <t>or</t> <t>ΔHBR</t> histone octamers, and DNA cleavage reactions were performed at 37 °C for the specified time with UDG (30 nm) and APE1 (10 nm). Cleavage activity was measured on denaturing gels (see insets), as described under “Experimental procedures.” The data points represent the means ± S.D. of at least three independent experiments.
Nt.Bstnbi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+wt+ape1/Nt%2EBstNBI/custom%40r0607%4026743004
Average 96 stars, based on 1 article reviews
nt.bstnbi - by Bioz Stars, 2026-10
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noti  (New England Biolabs)
99
New England Biolabs noti
Effect of HBR deletion on uracil removal by <t>UDG-APE1.</t> 601 DNA, containing a single uracil at the specified locations, was 5′-end–labeled with either [γ-32P]ATP or Cy3 and annealed with the corresponding undamaged complementary strand. Substrates were reconstituted with WT <t>or</t> <t>ΔHBR</t> histone octamers, and DNA cleavage reactions were performed at 37 °C for the specified time with UDG (30 nm) and APE1 (10 nm). Cleavage activity was measured on denaturing gels (see insets), as described under “Experimental procedures.” The data points represent the means ± S.D. of at least three independent experiments.
Noti, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+wt+ape1/NotI/custom%40r0189%4026743004
Average 99 stars, based on 1 article reviews
noti - by Bioz Stars, 2026-10
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Image Search Results


Effect of HBR deletion on uracil removal by UDG-APE1. 601 DNA, containing a single uracil at the specified locations, was 5′-end–labeled with either [γ-32P]ATP or Cy3 and annealed with the corresponding undamaged complementary strand. Substrates were reconstituted with WT or ΔHBR histone octamers, and DNA cleavage reactions were performed at 37 °C for the specified time with UDG (30 nm) and APE1 (10 nm). Cleavage activity was measured on denaturing gels (see insets), as described under “Experimental procedures.” The data points represent the means ± S.D. of at least three independent experiments.

Journal: The Journal of Biological Chemistry

Article Title: A cassette of basic amino acids in histone H2B regulates nucleosome dynamics and access to DNA damage

doi: 10.1074/jbc.RA117.000358

Figure Lengend Snippet: Effect of HBR deletion on uracil removal by UDG-APE1. 601 DNA, containing a single uracil at the specified locations, was 5′-end–labeled with either [γ-32P]ATP or Cy3 and annealed with the corresponding undamaged complementary strand. Substrates were reconstituted with WT or ΔHBR histone octamers, and DNA cleavage reactions were performed at 37 °C for the specified time with UDG (30 nm) and APE1 (10 nm). Cleavage activity was measured on denaturing gels (see insets), as described under “Experimental procedures.” The data points represent the means ± S.D. of at least three independent experiments.

Article Snippet: UDG and APE1 cleavage measurements NCPs (20 n m ) containing either WT or ΔHBR recombinant histone octamers were treated with E. coli UDG and human APE1 (New England Biolabs) at concentrations shown in the figures.

Techniques: Labeling, Activity Assay